Hi Kent,
Q: Do the tubes need to warm up before they sound their best? If so, about how long?
The Isabella features an auto-mute delayed turn-on sequencing (approx. 40 seconds) to allow for adequate tube warm-up and stabilization and to eliminate turn on/off transients. However, I have found that it is best to wait 5 to 10 minutes before critically listening. After that, the tubes should reach their peak temperature and everything should be in equilibrium.
Q: This one will probably be in the manual, but what is the recommended break-in period?
Yes, per the manual:
While your Isabella line-stage preamplifier will sound very good right out of the box, before critically evaluating its performance we highly recommend allowing for at least 100 hours of total use (burn-in time) connected to your amplifier (in order to load the output) and playing music. A sonic change will occur during this period that is the result of electrons flowing through the entire signal path of your Isabella. The dielectric of the various capacitors will form with use, and the included tubes need time burn-in as well. Q: How many hours do these types of tubes operate before they need replacement?
You should get
plenty of hours on them (a few years at the very least)... we are not running them on high B+ voltage, so they are being treated like gold in the Isabella

Q: What about tweaks like tube rings (or what ever they're called that people put on tubes)? Do they help the sound? If so, which brands / types should we be considering?
I haven't played with tube rings like the Herbies yet - thing may or may not sound better with these things (it just might sound "different" but not necessarily better).
NOTE: The distance between the two tubes is no greater than 5/16", and the distance between the rear tube and the jacks behind it is 1/4", so certain tube rings and other tube treatments might not fit.
Q: I assume that tubes are more sensitive to vibration than the Sig 30, any recommendations for isolation or vibration control?
The Isabella (as well as the RWA amps) is NOT very sensitive to vibration. Feel free to try different vibration tweaks and racks, but they sound fanstastic even stacked on each other!

Q: What else should a newbie to tubes know about the Isabella to get the best sound and longest life out of the unit???
Just sit back and enjoy it! If you want to try some NOS tubes, I think you'll have fun and they do make a difference - but the Isabella still sounds great with the stock tubes.
Hmmm...
Being battery operated, I was kinda hoping the RWA gear would be more immune to the vibration / isolation considerations. And with such a small footprint it would seem a shame not to be able to just stack em and save space rather than have to space them out in a rack.
Did it really make that big a difference? I suppose side by side might be another option.
Hi Kitten,
Yes, you can stack them (I recommend putting the Isabella on the top) - they sound just fine like this, and you can even try using small cones between the units stacked, and under the bottom unit.
Best regards,
Vinnie