Nelson26, in general you want the inside of a sealed box to be acoustically quite absorptive but with a vented box you don't want to overdo the stuffing material as that can kill the bass.
If you're building a sealed box then I'd suggest using about 1/2 pound per cubic foot of Polyfill, avaliable at fabric stores.
If you're building a vented box, stuff about half of the box with 1/2 pound per cubic foot Polyfill and leave the other half of the box unstuffed. Keep the stuffing away from the port(s) - in other words if the port is in the top rear of the cabinet, stuff the bottom half but leave the top half unstuffed.
As an alternative, with a reflex enclosure glue empty cardboard egg cartons to the inside surfaces wherever you can, and use little or no stuffing. You want the egg-holding side out so that the sound bouncing around inside the cabinet sees a highly diffusive surface when it hits an interior wall. Gorilla glue works good for this. You can also glue convoluted foam to the interior walls, but I suggest only on one of each pair of opposing walls (like do one side wall, the back wall, and the top). Acousstic fiberglass is an alternative, but it's nasty nasty stuff that I wouldn't wish on anybody.
My preference for dealing with panel resonances is bracing and/or contrained layer damping. The latter is where you glue large squares or rectangles of plywood to the inside surfaces using a glue that stays soft. I think there are some types of Liquid Nails that have this property, but I use a water-based glue that I buy in quantity. If you ask at the paint department at Home Depot for a glue that stays soft they can probably help you.
Feel free to call or e-mail me if you'd like, and best of luck with your project.
Duke LeJeune
audiokinesis at yahoo dot com
208-852-2610