Assuming the cable doesn’t have arrows printed on it, just go for it.
You might need time for them to burn in again, just from physically moving them, but any perceived deficiencies in sound will most definitely pass, and they will perform just as before. Possibly even better. With this in mind, hot-swapping direction to test is pointless.
I recently bought $2000 of very fancy used cables, and at no point was direction discussed. But all my cables here do take a few hours to bed-in again after simply being moved. Not surprising since both my speaker cables and interconnects are dozens (48) of individually double-jacketed silver-alloy runs on both hot and negative.
Just make sure you wire hot and negative to the same terminals as before, if the terminations aren’t colour-coded.
P/S - Removing the 12ft cables from under the floor, will at least give you the chance to inspect them fully for rodent bite-damage, and acidic waste-product staining. Not to mention the chance to flush out possible illegal immigrant infestation.