Yes.
Input impedances combined of valve amp (usually very high) and active xover must be high enough not to affect frequency response. I doubt it will be a problem. You will need a RCA to XLR lead, I make up my own, have a look at TNT's XLO type DIY leads.
I use a 3 way active xover, it is so transparent and adjustable, if I change drivers I can adjust xover point, levels and balance all day long! Sometimes I even listen to music. I also change resistors, caps, ICs, sockets, potentiometers, wiring, metalwork, power supply, and house wiring.
Sometimes I even listen to music! My (standard) TVC lets me listen at very low levels, as it is very late when I have finished changing everything around.