I had the same question a short while ago about my U-frames using a 21" woofer from MCM. MJK suggested a U-frame depth of between 5.5 and 6.5 inches, not counting the baffle. See the thread: "How do I determine the optimum U-frame depth", not too far down the page. My U-frames were originally 18" deep (I don't remember how I came up with that depth), with stuffing in the back, but after I cut them down and took out the stuffing, they sounded much better. Waaaay better. My U-frames are 24" square outside dimension, with 3/4" ply sides and a 3/4" baffle. 22.5" inside dimension. Very close to what you are considering. Based upon MJKs advice, I think 16" is too deep, and I am not sure stuffing the back actually affects very low frequencies. Maybe read that thread and try a baffle depth based upon his advice.